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cosmid the type of artificially constructed vector taken in use for cloning the 35-45 kb of dna these are plasmids carrying the phage l cos site
contig number of uses all nouns the term comes from the shortening of the word contiguous a contig might refer to the map showing placement of a
conservative substitution the nucleotide mutation that alters the amino acid sequence of protein but which causes substitution of one amino acid with
codon bias is the tendency for an organism or virus to make use of certain codons more than the others to encode a particular amino acid a
codon is the group of three consecutive nucleotides within the messenger rna mrna which encodes a message to initiate translation to incorporate
coding sequence is the portion of the gene or the mrna which actually codes for a protein introns are not coding for the sequences nor are the 5 or 3
cloning vector the dna molecule originating from the virus a plasmid or the cell of a higher organism into which an another dna fragment of
clone verb is the action of duplicating the genetic material within a vector to clone the piece of dna one would insert it into some type of the
chromosome walking is the technique for cloning everything in genome around the known piece of dna the begning probe you screen the genomic
chromosome jumping is the technique whereby one begins with a piece of dna from one area of a chromosome and obtains clones from nearby areas
chromosomes are the condensed fibrillar self- replicating genetic structures of cells containing the cellular dna which bears in its nucleotide
centimorgan cm is the unit of measure of a statistical probability recombination frequency between the alleles one centimorgan is equal to the 1
cat assay is an enzyme assay cat stands for the chloramphenicol acetyl transferase a bacterial enzyme which inactivates the chloramphenicol by
cap site two usages are given below1 in eukaryotes the cap site is position in the gene at which transcription begins and really should be called the
blotting is the technique for detecting one rna within the mixture of rnas a northern blot or one type of the dna within of a mixture of dnas a
binding site is the place on cellular dna to which a protein like transcription factor can bind typically binding sites may be found in the
bacteriophage is a virus which infects a bacterium and which is many times used in molecular genetics experiments as the vector or cloning vehicle
bacterial artificial chromosome bac is a chromosome-like structure made by genetic engineering bac is a cloning vector able to carrying between
autoradiography is the process to detect radioactively labeled molecules which commonly have been separated in an sds-page or agarose gel based on
atg or aug the codon for the methionine the translation initiation codon generally protein translation can only start at the methionine codon
arrayed library an individual primary recombinant clones hosted in the phage cosmid yac or other vector which are placed in two- dimensional arrays
antibiotic resistance is the resistance conferred to the host the ability to survive a given antibiotic by the plasmids containing resistance
amino acid is any of a class of 20 molecules which are combined to form the proteins in living things consisting of the general formula nh2-chr-cooh
allele-specific ligation is the technique permitting discrimination of two allele at locus by giving two short synthetic oligonucleotides which
alkaline phosphatase is an enzyme which catalyzes the hydrolysis of the phosphomonoesters of the 5 nucleotides it is used to dephosphorylate