Start Discovering Solved Questions and Your Course Assignments
TextBooks Included
Active Tutors
Asked Questions
Answered Questions
inverse pcr is the variation of pcr which makes the amplification of dna segments of unknown sequence that flank dna segments of the known
a kinase is in general an enzyme which catalyzes the transfer of the phosphate group from atp to something else in the molecular biology it has
knock-out experiment is a method for deleting mutating or otherwise inactivating the gene in a mouse this laborious technique involves
messenger rna mrna are the proteins are not synthesized directly from the genomic dna insteadof that an rna template a precursor mrna is
monoclonal antibody is an antibody with quite specific and often unique binding specificity which is secreted by the biologically cloned line of
northern blot is a technique for analyzing mixtures of rna by transfer of size-separated rna fragments to a synthetic membrane the presence and
nuclear run-on is a technique used to estimate the relative rate of the transcription of a given gene as opposed to steady-state level of the mrna
nuclease is an enzyme which degrades the nucleic acids a nuclease can be dna-specific a dnase rna-specific rnase or non-specific it might act only
oncogene is the gene in a tumor virus or in the cancerous cells which when transferred into the other cells can cause the transformation note
phosphorylation is the addition of the phosphate monoester to a macromolecule catalyzed by a particular kinase enzyme with respect to the proteins
physical map is the map of locations of the identifiable landmarks on dna for example restriction of the enzyme cutting sites genes regardless of
plasmid is the class of the circular extrachromosomal autonomously replicating dna elements found in number of bacteria contain origins of the
glycosylation the covalent addition of the sugar moities to n or o atoms present in the side chains of various amino acids of certain proteins
genomic clone is the piece of a dna taken from genome of a cell or an animal and spliced into the bacteriophage or other kind of cloning vector a
genomic blot is the type of southern blot particularly used to analyze the mixture of dna fragments derived from total genomic dna because the
genome is the whole compliment of genetic material in the form of the permanently maintained dna for the particular given organism its size is
gene is the fundamental physical and functional unit of the heredity generally dna some of the organisms have rna a gene a gene is an ordered
gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific dna
flow cytometry is an analysis of biological material by detection of light- absorbing or fluorescing properties of cells or subcellular fractions
expression clone is a clone plasmid in the bacteria or might be a lambda phage in bacteria which is designed to produce the protein from the dna
to express a gene is to make it to function properly a gene which encodes a protein will when expressed be transcribed or translated to produce that
expressed sequence tag est is the short part of dna sequence of corresponding to the fragment of the complementary dna made from the cells
exon are those parts of a genomic dna sequence which will be represented in final mature mrna t a contiguous segment of the genomic dna which codes
ethidium bromide intercalates within structure of the nucleic acids in such a manner that they fluoresce under the uv light ethidium bromide
epitope as related to the protein antigens b-cell epitopes comprises the amino acid residues of the protein molecule in which interact directly