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physical map is the map of locations of the identifiable landmarks on dna for example restriction of the enzyme cutting sites genes regardless of
plasmid is the class of the circular extrachromosomal autonomously replicating dna elements found in number of bacteria contain origins of the
glycosylation the covalent addition of the sugar moities to n or o atoms present in the side chains of various amino acids of certain proteins
genomic clone is the piece of a dna taken from genome of a cell or an animal and spliced into the bacteriophage or other kind of cloning vector a
genomic blot is the type of southern blot particularly used to analyze the mixture of dna fragments derived from total genomic dna because the
genome is the whole compliment of genetic material in the form of the permanently maintained dna for the particular given organism its size is
gene is the fundamental physical and functional unit of the heredity generally dna some of the organisms have rna a gene a gene is an ordered
gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific dna
flow cytometry is an analysis of biological material by detection of light- absorbing or fluorescing properties of cells or subcellular fractions
expression clone is a clone plasmid in the bacteria or might be a lambda phage in bacteria which is designed to produce the protein from the dna
to express a gene is to make it to function properly a gene which encodes a protein will when expressed be transcribed or translated to produce that
expressed sequence tag est is the short part of dna sequence of corresponding to the fragment of the complementary dna made from the cells
exon are those parts of a genomic dna sequence which will be represented in final mature mrna t a contiguous segment of the genomic dna which codes
ethidium bromide intercalates within structure of the nucleic acids in such a manner that they fluoresce under the uv light ethidium bromide
epitope as related to the protein antigens b-cell epitopes comprises the amino acid residues of the protein molecule in which interact directly
the enhancer is the cis-acting nucleotide series to which the transcription factors bind and which increases transcription of the gene it is not part
electrophoresis is the technique of separating the large molecules for instance dna fragments or proteins from the mixture of identical molecules
dot blot is the technique for measuring the amount of one perticular dna or rna in the highly complex mixture the samples are spotted onto the
dna deoxyribonucleic acid is the molecule which are responsible for storing and transmitting the genetic information dna is a double- stranded
dideoxy sequencing is the enzymatic determination and consideration of dna or rna sequence by the rechnique of sanger and colleagues based on
denaturing gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or rna for protein this
denaturation with respect to the nucleic acids refers to the conversion from the double-stranded to single-stranded state many times achieved by
database search once an open reading frame or the partial amino acid sequence has been pre-determined the investigator compares sequence with others
alzheimers disease - a devastating brain disease which gradually ruins the thinking skills