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define pipette - nutritional biochemistrypipettes are used to deliver a known volume of liquid from one container to another they are precision
define burette - nutritional biochemistrya burette is a vertical cylindrical piece of laboratory glassware with a volumetric graduation on its full
define volumetric flasks - nutritional biochemistrya volumetric flask is used to make up a solution of fixed volume very accurately it is calibrated
define oven - nutritional biochemistryoven is a general term used to describe a chamber or enclosed compartment for heating baking or roasting food
explain about the spectrophotometerthe spectrophotometer a key instrument today in biomedical laboratories was invented in 1939 by the american
explain about the colorimetercolorimeter is an instrument for measuring the colour or colour intensity of a solution it is an instrument that
define the water bath with shakeran electric water bath with a shaker that can hold test tubes as well as conical flasks when on a non-shake mode it
explain about the autodispensorthis is used to transfer accurate quantities of liquids that are difficult to pipette like concentrated acids alkalis
define the working of cyclomixerthis is an electrical mixer used for mixing the contents of a test tube the speed of the mixer can be adjusted to
define the working of ph meterthe ph of a solution we know measures the degree of acidity or alkalinity relative to the ionization of water sample a
basic instruments equipment used in biochemical laboratories and important working tipsthe first practical in this manual will orient you to the
define precautions for determination of haemoglobin content in blood1 potassium cyanide is highly toxic and hence drabkins solution should not be
define sample preparation for haemoglobin content in blood wipe the index finger or middle finger with a cotton swab soaked in spirit prick the
preparation of the standard curve for haemoglobin label 6 clean dry rest tubes and label them as s1 -s6 add 5 ml of drabkins solution to test tube
define cyanmethaemoglobin standard - haemoglobin content in bloodcertified standard haemoglobin solution may be obtained from certified or reputed
define ferricyanide-cyanide reagent or drabkins solutiondissolve 02 g potassium ferricyanide 005 g potassium cyanide and 10 g sodium bicarbonate in
define reagents estimation of iron in the solution1 conc sulphuric acid iron free2 conc potassium permanganate solution3 saturated potassium
define precautions for determination of inorganic phosphorus1 all reagents should be added in the order mentioned2 allow 10 minutes for the colour to
define regents for determination of inorganic phosphorus1 trichloroacetic acid 10 - dissolve 100 g of high grade crystalline acid in distilled water
define reagents for estimation of calcium using edta titrimetric method1 buffer solution dissolve 169 g nh4ci in 143 ml nh4oh to this add 50 ml of
define the principle behind cyanmethemoglobin methodthe principle behind the cyanmethemoglobin method is as followprinciple the haemoglobin is
define the estimation of haemoglobin in bloodseveral methods are used to determine haemoglobin in these methods hemoglobin is measured as
define estimation of iron in any solutioniron can be determined calorimetrically the principle is described nextprinciplethe iron is determined in
define methods for estimation of iron and haemoglobinin this section we shall study the procedure involved in estimation of iron in any sample first
define iron - the micronutrientiron as you may be aware is a trace element present in the body the body contains approximately 3 - 35 g of iron of