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estimation of nitrogen and protein content using micro kjeldahls methodstandardization of h2so4prepare a solution of 01 n h2so4 by diluting 28 ml
define precaution for separation of amino acids by paper chromatography1 hold the paper with the extra strip kept at one side handling of whatman
explain procedure for separation of amino acids by paper chromatographynow carry out the procedure following the steps given herewith1 take a
define reagent for separation of amino acid by paper chromatographythe following reagents will be required for carrying out the experiment1 amino
determine materials for separation of amino acids by paper chromatographypetridishes 200 mm diameter with lid whatman chromatographic paper brown
explain about the biuret methodthe biuret reaction was one of first colorimetric assays developed for protein estimation it is most often used in
determine how to calculate the amount of nitrogenthe amount of nitrogen can be calculated for this value using the equation1000 ml of 1n h2so4 1000
explain methods of protein estimationseveral methods for protein estimation are available these methods are based on several analytical
define amino acids in the building blocks of proteinsamino acids as you have already illustrated have a carboxyl group and an amino group bonded to a
explain about the amino acids and proteinswe shall now study the properties of amino acids which we know are the building blocks of proteins in
explain precautions for estimation of reducing sugar by fehling soxhlet method1 clamp the burette so that the tip of the burette is exactly above the
define sample titration for fehling soxhlet methoddilute the given sample glucose solution in 100 ml volumetric flask to the mark with distilled
define standard titration for estimation of reducing sugar by fehling soxhlet methodpipette 5 ml of fehlings a and 5 ml of fehling b solution into a
explain preparation of burette for estimation of reducing sugar by fehling soxhletpreparation of burette - take a 50 ml burette1 fix the burette into
define regents for estimation of reducing sugar by fehling soxhlet method fehling a solution - copper sulphate solutionfehling b solution - alkaline
explain precautions for estimation of sucrose by fehling soxhlet method1 maintain proper temperature of the water bath for complete hydrolysis2 add
explain procedure for estimation of sucrose by fehling soxhlet methodfollow the proceedure enumerated herewith and carry out the experiment1 standard
procedure for define percentage of reducing and total sugar by fehling soxhlet1 standard titration standardization of fehlings solution - titrate 10
principle for estimation of percentage of reducing and total sugar by fehling soxhlet methodreducing sugars are those which have free sugar groups
explain procedure for nelson somogyi method for glucose estimationcarry out the procedure following the steps enumerated herewith1 label a series
define reagents - nelson somogyi method for glucose estimation1 alkaline copper reagentalkaline a- buy commercial from sds chemicals etcalkaline b-
define procedure for determination of blood glucose by nelson somogyi method1 label a series of folins tubes with blank b standard s1-s5 and sample
define principle for determination of blood glucose by nelson somogyi methodproteins are precipitated from the sample and protein free filtrate is
define reagent for determination of blood glucose by nelson somogyi 1 alkaline copper reagenta dissolve 15 g of sodium potassium tartarate and 30 g
define practical preparation - qualitative test for monosaccharides1 wear your labcoat make sure your hair is tied and you have no loose clothing