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define the starch gel electrophoresisstarch gel has the advantage of fine texture and absence of protein adsorption with a molecular sieving effect
define about the cellulose acetate electrophoresis caeif the hydroxyl groups in cellulose are reacted with acetic anhydride cellulose is acetylated
define the agarose gel electrophoresis agethis technique is used to analyze serum proteins haemoglobin variants lactate dehydrogenase isoenzymes
define the gel electrophoresisgel electrophoresis is among the most powerful and yet conveniently used methods of macromolecular separation the gels
define about the paper electrophoresisin paper electrophoresis the sample is applied to a point on a strip of filter paper or cellulose acetate
define role of buffer in electrophoresisbuffer ions have a two-fold purpose in electrophoresis they carry the applied current and they fix the ph at
explain about the zone electrophoresiszone electrophoresis which was introduced about a decade later describes the migration of charged
define free solution or moving boundary methodthe first electrophoresis technique used in the study of protein was free solution or moving boundary
explain about the electrophoresiselectrophoresis refers to the movement of charged particles because of an external electric field because charged
define efficiency - terminologies used in chromatographyefficiency in chromatography is expressed as number of theoretical
define selectivity - terminologies used in chromatographyselectivity is a measure of preference a stationary phase shows for one solute over another
define resolution - terminologies used in chromatographyresolution is the effectiveness of the separation and is determined by two factorsthese
distribution coefficient - terminologies used in chromatographyduring the purification or separation of the biomolecules it should be kept in mind
define development - terminologies used in chromatographydevelopment is the process of passing a mobile phase over the adsorbent or support during
define gas liquid chromatographythis is used for separating and identifying volatile compounds of fatty acids hydrocarbons sterols amino acids and
define thin layer chromatographythis is an improvement over paper chromatography and uses thin layers of slurried absorbents like silica gel alumina
why ascending method of paper chromatography is preferredthe ascending method is preferred because of the simplicity of the set up in actual method
define descending type of paper chromatographyin descending chromatography the movement or flow of mobile phase is in downward direction in this
define ascending type of paper chromatographythe ascending type consists in dipping the lower end of the paper containing the sample spots into the
explain following trems a state three structural features of prokaryotic cellsb describe the functions in eukaryotic cells of lysosomes ribosomes and
define technique of paper chromatographypaper chromatography is used as1ascending 2descending or3horizontal technique circular paper chromatographyit
define about the paper chromatographypaper chromatography is a very useful technique for separating mixtures of metal ions anions amino acids sugars
define about the column chromatographyin column chromatography a tube is filled with the material constituting the stationary phase plus a solvent
define modes or techniques for chromatographic separationin practice chromatographic separation may take one of these modes or techniques paper
define ion exchange chromatographyin this method a reversible exchange of ions is effected usually resins and ionic solutes are the two components