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define sample collection and estimation of blood glucosethe aim of blood glucose estimation is to diagnose or to exclude or to monitor the
explain the principle of alkaline copper reduction methodall sugars which contain a free aldehyde group undergo enolization when placed in hot
explain the alkaline copper reduction methodsince alkaline copper reduction methods are by far the most commonly used methods for blood glucose
determine some methods of blood glucose estimationthe various types of methods for estimation of blood glucose can be grouped under two groupsa
explain the principle of nelson-somogyi methodglucose is estimated by nelson-somogyi method we begin our study of this method by getting to know the
explain the colorimetric method glucose estimationalkaline copper reduction methods are by far the most commonly used methods for glucose estimation
explain principle of fehlings soxhlet method lane-eynon methodreducing sugars are those which have free sugar groups and hence may be estimated
define the fehlings soxhlet method lane-eynon methodthis is a titrimetric method that is commonly used in food laboratories to estimate percentage of
define titrimetric estimation of carbohydratesthe fehlings soxhlet method is the commonly used method for titrimetric estimation of carbohydrates in
define quantitative procedures in carbohydratesyou have learnt how to identify some individual monosaccharides disaccharides and polysaccharides
define key concepts and facts of polysaccharides1all polysaccharides contain several monosaccharide units2all monosaccharide units are joined to each
define key concepts and facts about disaccharides1all disaccharides contain 2 monosaccharides2disaccharides are linked by glycosidic linkage3if both
define key concepts and facts on properties of monosaccharides1all monosaccharides are soluble in water2all monosaccharides give molischs
define observation or inference for half and full saturation test1 starch is precipitated by half saturation with ammonium sulfate whereas dextrin is
define methodology for half and full saturation test1 take 5 ml each of starch and dextrin solutions add 5 ml of saturated solution of ammonium
define half saturation test and full saturation testthis test is specific to polysaccharides the test is used to detect dextrin from starch the
explain observation or inference required for osazone test1 crystals of different shapes will be seen glucose and fructose give needle shaped
explain methodology required for osazone testtake about 250-300 mg of phenylhydrazine mixture in the 3 clean labelled 15 ml dry test tubes add 5 ml
define reagents required for osazone test- solutions of glucose galactosefructose lactose maltose sucrose- phenyl hydrazine mixture 2 parts of
explain principle osazone test or phenylhydrazine reactionphenylhydrazine reacts with carbonyl compounds in neutral or slightly acidic medium to give
define the osazone test or phenylhydrazine reactioncompounds with -co-choh group form crystalline osazones with phenyl hydrazine these osazones have
define observation or inference for mucic acid test1 white crystals of mucic acid will be seen2 the sugar solution contains galactose3 lactose gives
define reagents required and methodology for mucic acid test- sugar solution- conc nitric acidmethodologytake about 50 mg of sugar in
what is mucic acid test and its principlethis is a specific test for galactose and is given by galactose as well as lactose which is made up of
explain the observation or inference for seliwanoffs test1 wine or cherry red colour seen wine red colour confirms the presence of a ketose sugarnote