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You are a forensic scientist and you perform a southern analysis to try and link a possible suspect to the scene of a crime. Draw the results of such a southern analysis and decide if the suspect wa
If you have a full-length cDNA clone that is a perfect copy of an mRNA species you ought to be able to predict the primary amino acid sequence of a translation product. For a mouse gene how would yo
If we do not move forward with either therapeutic or human cloning, perhaps we're preventing ourselves from making new revolutionary scientific discoveries.
Researchers are unable to guarantee that their work with stem cells will produce beneficial results. How should this uncertainty influence the debate on whether scientists should be allowed to condu
Engineering a recombinant plasmid DNA(like GST- preferably use other than GST)to over-express the protein encoded by the MsrB gene from Drosophila, by cloning it into the pGEX vector.
The United Nations realizes that at some future date, some government will be prepared to pay for human cloning in order to develop professional athletics who have been scientifically bred and engin
What is the difference between reproductive and therapeutic cloning? Name one limitation for each technique. Make sure to differentiate between pluripotent and totipotent embryonic stem cells as wel
Three recessive mutations in DROSOPHILA cause black body color(b), dumpy wings(dp), and hooked bristles(hk). From a cross of dumpy female with a black, hooked male, all the F1 were wild type for all
Time of entry mapping between a streptomycin-sensitive, prototrophic Hfr strain and a streptomycin- resistant Fminus strain that was Trp- Pyr- Cys- Nic- did not allow the order of the trp,pyr,cys ge
In guinea pigs, black (B) is dominant to brown (b), and dolid color (S) is dominant to spotted (s). A heterozygous black, solid-colored pig is mated with a brown, spotted pig. The total offspring fo
Which example below is an example of allelic frequency.
Explain the process of creating a recombinant microorganism.
Explain how differential gene expression yields a variety of cell types in a multi-cellular eukaryote.
In this experiment, we will change the genotype and therefore the phenotype of a bacterium by genetically transforming an organism by introducing foreign DNA into its genome. This is called transfor
In recent years, molecular biology has undergone a revolution that can be partially attributed to the advent of easy DNA sequencing methods.
If DNA and a base were inserted at the beginning of the 5' end of the molecule, how would that affect protein synthesis and the resultant protein? (Hint: think about the code)
Each of the following pairs of primers have a problem with it. Tell why the primers would not work well.
Describe how you would use site-directed mutagenesis to change a BamHI restriction site into an EcoRI site.
If a DNA polymerase other than the polymerase from Thermus aquaticus was used in the PCR process, what would happen.
Electrophoretic mobility, describes the velocity of a particle moving through a gel matrix under the influence of an electrical field. Given a constant electrical field.
What happened to Rosalind Franklin with respect to her career following the discovery of the chemical nature of DNA.
Compare the DNA Replication process in cells with the process occurring in the PCR replication.
Discuss different models for DNA replication that were considered by early researchers. Explain why they settled on the current model for DNA replication. Describe this model in detail and interpret
Genetic variability is beneficial for a species because it enhances its ability to adapt to changing conditions. Why then does a cell go to great lengths to assure the fidelity of DNA replication.
Can you think of a way in which you could inhibit this repair mechanism in bacteria that have been exposed to UV radiation.