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Write down the components of an operon? What significant regulator is not part of the actual operon? Know how operons are classified and how each kind works (Inducible, Repressible, Positive and Neg
The promoters for mRNA encoding early proteins in viruses similar to T4 have a dissimilar sequence than the promoters for mRNA encoding late proteins in the same virus.
Compare and contrast the repressive effects of chromatin and DNA modifications with the repressive effects of RNAi. Comprising: a) A fundamental overview of RISC and RITS repression.
Write down the advantages and drawbacks of using random transgenic integration compared with the homologous recombination to introduce DNA into the genome?
What are the merits to a microbe as an effect of such (inducible) an arrangement of genes, and what is this arrangement termed?
Give two key insights into the evolutionary history of Neandertals which have been derived from the analysis of mitochondrial DNA (mtDNA). In what manners might the use of mtDNA be misleading or lim
Explain how does transcription distinct from DNA replication? How is it similar? Describe the role of complementary base-pairing in each?
By using the three criteria outlined in the problem decide whether the experimental outcomes in the presence of microsomes (lanes 5 to 8) point out that the protein is translocated across microsomal
The galactose represser protein from E. coli has a pI of around 5.9. While purification protocols were being designed, and it was found to bind to a Mono-S column at pH values of 7 and below.
Write down the function of "stress" proteins under heat shock conditions and explain how is this associated to their function under normal conditions? I describe the method underlying their actions
A strand of DNA has the base sequence GCTTAG. Write down the base sequence for the complementary strand. Keep in mind that the DNA and RNA strands are written from the 5' to the 3' end.
Name the function which does the "leader sequence" have in RNA translation. What would happen if it were missing? Does this protein require a leader sequence?
DNA (3.5 x 108 base pairs) in diploid cells of Drosophila melanogaster is replicated in around 3 minutes. Supposing that all replication forks move at a constant rate of 104 base pairs per minute an
When a single polypeptide chain protein has 185 amino acids, then what is the smallest number of mRNA nucleotides which could code for this protein? Explain how is this computed?
Will a change in a single DNA nucleotide (that is, a nucleotide substitution mutation) always outcome in a changed protein? Explain why or why not?
1) Explain, in general terms, the steps comprised in the automated synthesis of the DNA sequence GCT. 2) Draw and label the four DNA nucleosides.
Describe two of these four principal organic molecules. Where do you encounter these groups in your day by day life, and describe their significance to your body.
Knowing the sequence of a protein, a scientist synthesizes the corresponding RNA. By using the machinery of a cell and this RNA, the scientist then re-synthesizes the protein.
Design a non-natural base which will pair with thymidine with around equivalent strength to a GC base pair. Show the pairing interaction. No de novo synthesis is given.
Pertaining to the purine biosynthesis pathway of the mammalian cells, write down one illustration of a drug which inhibits a specific step of this pathway, the method of action, and how the drug is
Explain the processes of transcription and translation. Correctly recognize the anticodon for the given DNA template strand: ACG TGG GCA TGA ACG TGG.
Compare and contrast the similarities, differences and functions of the DNA and RNA.
Explain why do we use 16s rDNA for sequencing? Explain why do we use the nanodrop on our DNA extracts?
a) Write down two distinguishing features of a tRNA molecule. b) In translation what does the ribosome do?
In an actin assembly and a microtubule polymerization, whenever an ATP/GTP cap is formed, what do you mean by "stabilizing" the microtubule or microfilament?