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establishment of suspension cultures a callus crumbles into smaller clumps and single cells in liquid medium by gentle agitation 100-120 rpm on a
characteristic of callusthe most important characteristic of callus from a functional view point is that abnormal growth has the potential to develop
establishment of tissue cultures by now you are familiar with the term explants after a few days in culture the explant becomes slightly rough in
culture conditionsthe culture vessels placed in suitable trays or racks are stored on the shelves in a hygienically maintained culture room under
general protocol for media preparationa general protocol for media preparation is as follows1 prepare stock solutions one day before the medium is to
preparation - culture mediumnow that you are familiar with the constituents the preparation of medium is quite simple several plant tissue culture
composition - culture mediuma standard plant tissue culture medium basal medium contains all the essential macro elements carbon hydrogen oxygen
culture medium - techniques of plant tissue culturein nature green plants are capable of synthesizing organic compounds necessary for their growth
initiation of aseptic culturesthe nutrient medium basal medium used for plant tissue culture would promote luxuriant growth of micro-organisms such
techniques of plant tissue culturea standard tissue culture laboratory should provide facilities for washing and storage of glass ware preparation
types of cambiumon the basis of the arrangement of the fusiform cells as seen in tangential section cambium is divided intoa storiedstratified
structure of the vascular cambiumthis meristem in the anatomical sense of the term usually includes two histologically distinct kinds of cellsi
vascular cambiumthe vascular cambium usually if not always has a dual origin within the primary tissues from provascular strands and from the ground
applications in horticulture and agriculture while going round in public parks you might have observed plants trimmed in such a way giving
ethylene - apical dominanceethylene also plays a role in the inhibition of lateral bud growth it is present in those tissues like shoot apex where
auxins - apical dominancethe role of auxins in controlling the apical dominance was shown by the experiments done by skoog and thimann with the broad
cytokinins - apical dominancecytokinins are also involved in the regulation of apical dominance wickson and thimann studied the interaction of
role of chemical factors in controlling apical dominance there were indications about the existence of plant hormones in the last part of 19th
terminology use in apical dominancehere are a few terms that will be used in discussing apical dominance a clear understanding of these terms is
apical dominanceapical dominance is a correlative phenomenon in the developmental biology of plants branching of the main shoot into lateral branches
molecular genetics and plant growth hormonemolecular genetics has advanced over the last three decades by leaps and bounds providing knowledge
tools and techniques - effects of plant growth regulators we have discussed about the effect of auxins in this section we shall discuss the
synergistic and sequential effects of hormonesin vitro studies on callus and suspension cultures have brought out two interesting findings the nature
abscission - effects of plant growth regulatorsabscission of leaves and fruit is one of the more obvious characteristics of senescence leaves do not
patterns of ageing and death plants and their parts develop continuously from germination until death the latter part of the developmental process