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production of industrial compoundscultured plant cells retain their metabolic potential and synthesise secondary products of commerce cell cultures
germplasm conservation - plant tissue and organ culturetotipotent plant cells and shoot tips can be freeze-preserved in liquid nitrogen -196 c for
merits of micro propagationthe special merits of micro propagation are1 it considerably increases the rate of multiplication2 high rate of
transplantation - clonal propagationthe shoots or plantlets multiplied on a medium containing organic nutrients show poor photosynthetic capability
shoot multiplication -clonal propagation this is the most important step with respect to the rate of propagation and genetic uniformity of the
clonal propagation most cultivars of ornamental and fruit species and forest trees are highly heterozygous consequently their seed progeny is not
androgenic haploidsandrogenic haploids of some species such as wheat mustard and tobacco can also be raised through isolated microspore pollen
production of disease-free plantsunder normal conditions plants are infected by a wide range of pathogens such as bacteria fungi viruses viroids and
haploid productionthe higher plants are normally diploid with two sets of chromosomes in their somatic cells their haploids with one set of
production of rare hybrids hybridization is a well established plant breeding procedure to obtain superior plants by combining useful characters
somatic embryogenesis the process of embryo development is called embryogenesis it is not the monopoly of the egg to form an embryo any cell of the
organogenesis - plant tissue and organ cultureorganogenesis refers to the differentiation of organs such as roots shoots or flowers shoot bud
cellular totipotency the innumerable cells which constitute the body of a higher plant or animal and containing identical genetic material can be
bergmanns method of cell platingin this method free cells are suspended in a liquid medium at a density twice the finally desired plating density
immobilisation of cellsimmobilisation of cells enables re-use of biomass by rotation of cells separation of cells from the medium and leaching of
establishment of suspension cultures a callus crumbles into smaller clumps and single cells in liquid medium by gentle agitation 100-120 rpm on a
characteristic of callusthe most important characteristic of callus from a functional view point is that abnormal growth has the potential to develop
establishment of tissue cultures by now you are familiar with the term explants after a few days in culture the explant becomes slightly rough in
culture conditionsthe culture vessels placed in suitable trays or racks are stored on the shelves in a hygienically maintained culture room under
general protocol for media preparationa general protocol for media preparation is as follows1 prepare stock solutions one day before the medium is to
preparation - culture mediumnow that you are familiar with the constituents the preparation of medium is quite simple several plant tissue culture
composition - culture mediuma standard plant tissue culture medium basal medium contains all the essential macro elements carbon hydrogen oxygen
culture medium - techniques of plant tissue culturein nature green plants are capable of synthesizing organic compounds necessary for their growth
initiation of aseptic culturesthe nutrient medium basal medium used for plant tissue culture would promote luxuriant growth of micro-organisms such
techniques of plant tissue culturea standard tissue culture laboratory should provide facilities for washing and storage of glass ware preparation