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applications of transgenic animalscurrent applications of gene transfer in farm animals include the improvement of product quality and quantity
equine infectious anaemiaequine infectious anemia eia an acute or chronic viral disease of the equines shows intermittent fever loss of weight
gene targeting in somatic cells since the pioneering work with sheep at roslin cloning by nuclear transfer has now been exemplified in a number of
african horse sicknessafrican horse sickness ahs is a highly fatal insect born viral disease of equidae caused by an orbivirus family reoviridae and
gene targeting in es cells in some of the livestock species pro-nuclear injection cannot be used to modify the animals own genes due to various
gene targeting there is always a debate on gene integration in to the host animalit is random gene integration vs gene targeting perhaps most
nuclear transfer method nuclear transfer will extend the range of species in which gene targeting will be possible and thereby provide better models
borna diseaseborna disease is fatal neurological disease of horses and sheep caused by a virus rna of the family bornaviridae and the disease is
embryonic stem cells es these are pleuripotent cells of early embryo blastocyst capable of giving rise to all differentiated cells including germ
microinjection method in another approach fertilised eggs are microinjected by dna microinjection requires excess of fertilized eggs gene transfer by
retroviral method a retrovirus is a virus that carries its genetic material in the form of rna rather than dna in this method retroviruses are used
methods of gene transferthe technology employed for introduction of transgenes into a livestock population may produce consequences specific to the
insulatorsnumerous experiments showed that a transgene is generally poorly expressed when it contains a cdna rather than the corresponding genomic
promotersthe promoter region contains the dna sequences that allow the formation of the transcription initiation complex that give the specificity of
design of expression vectorsexpressing a transgene in an appropriate manner is a huge task the gene construct that is quite active in transfected
gene constructsdesigning and cloning of the gene construct is the fundamental step in the generation of transgenic animals essentially determining
choice of transgenic animalfirst and foremost is the selection of animal species in which gene of interest is to be transferred whatever is the goal
transgenic animalsthere are various definitions for the term transgenic animal the transgenic animal is an animal in which there has been a
equine encephalomyelitisencephalomyelitis or inflammation of the brain tissue and the spinal cord can be brought about by different viruses eastern-
transgenic animalover the centuries animals with new combinations of genes have been produced using conventional breeding methods by means of careful
porcine rotaviruspiglet diarrhoea and mortality are gaining importance and the viral agent which is highly incriminated in such episodes is porcine
swine influenzaswine influenza a highly contagious disease affecting the respiratory tract of pigs is c a use d b y a vir us b e lo nging t o
aujeszkys diseasethis is primarily a disease of pigs which serve as the principal reservoir host the secondary hosts include cattle horse sheep goat
swine-poxpigs of 2 months of age may be infected with vaccinia either naturally or artificially and show pox lesions on the eyelids snot inside the