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gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific dna
flow cytometry is an analysis of biological material by detection of light- absorbing or fluorescing properties of cells or subcellular fractions
expression clone is a clone plasmid in the bacteria or might be a lambda phage in bacteria which is designed to produce the protein from the dna
to express a gene is to make it to function properly a gene which encodes a protein will when expressed be transcribed or translated to produce that
expressed sequence tag est is the short part of dna sequence of corresponding to the fragment of the complementary dna made from the cells
exon are those parts of a genomic dna sequence which will be represented in final mature mrna t a contiguous segment of the genomic dna which codes
ethidium bromide intercalates within structure of the nucleic acids in such a manner that they fluoresce under the uv light ethidium bromide
epitope as related to the protein antigens b-cell epitopes comprises the amino acid residues of the protein molecule in which interact directly
the enhancer is the cis-acting nucleotide series to which the transcription factors bind and which increases transcription of the gene it is not part
electrophoresis is the technique of separating the large molecules for instance dna fragments or proteins from the mixture of identical molecules
dot blot is the technique for measuring the amount of one perticular dna or rna in the highly complex mixture the samples are spotted onto the
dna deoxyribonucleic acid is the molecule which are responsible for storing and transmitting the genetic information dna is a double- stranded
dideoxy sequencing is the enzymatic determination and consideration of dna or rna sequence by the rechnique of sanger and colleagues based on
denaturing gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or rna for protein this
denaturation with respect to the nucleic acids refers to the conversion from the double-stranded to single-stranded state many times achieved by
database search once an open reading frame or the partial amino acid sequence has been pre-determined the investigator compares sequence with others
alzheimers disease - a devastating brain disease which gradually ruins the thinking skills
cosmid the type of artificially constructed vector taken in use for cloning the 35-45 kb of dna these are plasmids carrying the phage l cos site
contig number of uses all nouns the term comes from the shortening of the word contiguous a contig might refer to the map showing placement of a
conservative substitution the nucleotide mutation that alters the amino acid sequence of protein but which causes substitution of one amino acid with
codon bias is the tendency for an organism or virus to make use of certain codons more than the others to encode a particular amino acid a
codon is the group of three consecutive nucleotides within the messenger rna mrna which encodes a message to initiate translation to incorporate
coding sequence is the portion of the gene or the mrna which actually codes for a protein introns are not coding for the sequences nor are the 5 or 3
cloning vector the dna molecule originating from the virus a plasmid or the cell of a higher organism into which an another dna fragment of