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explain the identification of fungi and yeastsin this we learnt about different staining techniques used to visualize bacterial cell morphology what
explain precautions for capsule staining in a culture1 never heat fix the smear this is because by heating shrinkage can occur which may create a
explain procedure for capsule staining in a culturewe have learnt that capsule staining can be undertaken either by negative staining or anthony
define some precautions for spore staining in a given bacterial culture1 do not allow malachite green to dry on slide2 decolourization should be done
explain procedure for spore staining in a given bacterial culturecarry out the exercise following the steps enumerated herewith1 take a clean
define precautions for acid fast staining1 do not allow carbol fuchsin to dry on heating2 maintain aseptic conditions3 decolourization should be done
explain procedure for acid fast stainingcarry out the exercise using the steps enumerated herewith1 label the clean non-greasy slides and make smears
explain precautions for gram staining of bacterial cultures1 heat fix the smear otherwise cells would be washed off2 fresh 24 hrs old culture should
explain procedure for gram staining of bacterial culturesnow carry out the exercise following the steps enumerated herewith1 prepare bacterial smear
define precautions for simple staining of bacterial cultures1 heat fix all the bacterial smears2 use clean grease free glass slides3 wash the stained
explain procedure for simple staining of bacterial culturesnow carry out the exercise following the steps given herewith1 prepare thin bacterial
define principle for simple staining of bacterial culturesin simple staining a single staining agent preferably a basic dye is used commonly used
explain requirements for simple staining of bacterial culturesculture 24 hours bacterial culture of e coli and bacillus on nutrient agar or in
define precautions for preparation of bacterial smear1 the slide should not be greasy2 hold the slide by their edges only3 label should not come in
explain the procedure for preparation of bacterial smearstart the exercise by following the steps enumerated herewith1 take properly washed and dried
explain the staphylococcus - characteristics of bacteriastaphylococcus - it is gram positive nonsporulating facultative anaerobic cocci present in
explain the bacillus - characteristics of bacteriabacillus - it is gram positive endospore former obligate aerobic or facultative aerobic rods
explain the pseudomonas - characteristics of bacteriapseudomonas are aerobic gram negative straight or slightly curved rods that are motile by polar
explain the proteus - characteristics of bacteriait is gram negative non-sporulating rod which is characterized by rapid motility peritrichous
explain the klebsiella - characteristics of bacteriait is gram negative non-sporulating non-motile and non-pigmented rod which is commonly found in
explain the escherichia - characteristics of bacteriait belongs to order enterobacteriales it is the best-studied organism present in the colon of
explain the disadvantages of fixationfixation has following disadvantages also these are highlighted herewithi it distorts the cells appearanceii
define the chemical fixation methodhere chemical fixatives containing ethanol acetic acid and formaldehyde etc penetrates the cell and inactivates
explain the heat fixation methodthis is the usual method here bacteria is fixed by gentle heating of air-dried bacterial film which results in
what do we mean by fixationfixation is the process of preserving internal and external structures of microorganisms it leads to the killing of