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Draw a restriction map of the plasmid. Label the respective restriction sites (H and E for HindIII and Eco RI respectively) and indicate the distances between each of the fragments.
Five different types of transposable elements including insertion sequences, composite transposons, replicative transposons, viral-like retroelements, and nonviral-like retroelements.
The lifespan of a Red Blood Cell is about 120 days. Assuming constant rates of RBC production, calculate the rate of RBC production needed to maintain constant cell number.
Why is the amount of bacteria in a urine sample as important as the type of bacteria. Using a volumetric loop is a semi-quantitative technique.
Why would you want to preform an experiment on enterics. Why is identification of bacteria important to our welfare. What can we expect to find.
For example do you now understand the concept behind DNA fingerprinting. Did you find it difficult to determine the father in the paternity suit or the rapist.
Assume that you have conducted a study of a food borne outbreak that occurred recently at the "Road Kill Bar and Grill" First, explain which type of epidemiologic study design you would have been mo
To measure the population density of Monarch butterflies occupying a particular park, 100 butterflies are captured, marked with small dot on a wing, then released, the next day another 100 butterfli
Red-green colorblindness is the most common form of colorblindness and is caused by two closely linked genes located near the tip of the human X chromosome.
Black coat color in Cocker Spaniels is governed by a dominant allele and red coat by a recessive allele; solid pattern is governed by the dominant allele of another gene and spotted pattern is reces
The enzyme papain is a thiol protease, using a mechanism similar to the mechanism used by chymotrypsin, except that there is an active site Cys in papain carrying out the catalytic role played by th
The pKa of that basic functional group on the enzyme is 8.0, and is the only pKa apparent on a plot of kcat vs. pH for the activity of this enzyme. The kcat of the fully active form of the enzyme is
A woman is heterozygous. Which one could produce an egg in which two X chromosomes carry an allele for the recessive trait color blindness: nondisjunction during meiosis 1 or nondisjunction during m
what must happen to the cell parts, especially the nucleus of amoeba Proteus in order for it to successfully reproduce to form two new amoebas
If solution bubbles over then place you water + phenolphthalein solution into a cup before bubbling exhaled air into it Standard solution:Number drops NaOH solution to restore pink color
Some fungi cells complete mitosis however they do not complete cytokinesis. This would result in A) large nuclei containing many chromatids B) large cells containing lots of nuclei C) many cells each
An enzyme that follows the MWC (also known as the concerted model) model for allostery has a T/R ratio of 300 in the absence of substrate. Suppose that a mutation were to reverse that ratio(T/R= 1/3
The pKa of that basic functional group on the enzyme is 8.0, and is the only pKa apparent on a plot of kcat vs pH for the activity of this enzyme. The kcat of the fully active form of the enzyme is
the molecular weights (for the hind III fragments) beginning with the fragments nearest the origin are shown below fragment # , size in kilobase pairs (kb)
how will increasing the number of yeast cells affect the rate of ATP production by the cell (in moles ATP/hr) if the sugar concentration in the experiment is held constant
What would happen to respiration if we add a buffer or base to the external environment of the bacteria (assume bacteria is in a closed environment like a tube) How would this affect the mechanism o
he human genome project initially predicted 100,000 genes due to the number of proteins found through western blots. When near completion, computer analysis of the sequence predicted only 30,000 gen
If we add 100 ul of 0.5 mg/ml ConA and 100 ul of 1 M mannose to 800 ul of a dilute suspension of red blood cells in ConA buffer, what should we expect to happen
You want to make one liter of a solution containing 10 mg/ml sodium phosphate, 10 mg/ml sodium chloride, and 10 mg/ml potassium nitrate. How much of each starting solution do you need to use, and ho
The autosomal recessive gene transformer (tra) mutation converts chromosomal females into sterile males. A female Drosophila that is heterozygous for the tra allele is mated with a normal male homoz