You work for a biotechnology company and your project is to

Question

You work for a biotechnology company and your project is to produce large quantities of the human protein Farabin in bacteria. What would be the correct order of steps for you to accomplish this?

1. Ligate the Farabin fragment into a plasmid vector with a lac promoter
2. Digest the Farabin gene and the vector with the same restriction enzyme
3. Add IPTG to antibiotic resistant cells
4. Derive a cDNA for the Farabin gene
5. Transform the ligation reaction into bacteria