You need to calculate ligation for your insert. You know that you have 3 ng/μL in your sample, how many picomols/μL do you have if it is a 1000 bp insert?
Include your converstions and calculations.
Tips:A base pair has an approximate molecular mass of 660 g/mol*bp.
- 109 ng = 1 g
- 1012 pmol = 1 mol