Question - You have a mixture of Glycine, Lysine, and Glutamic acid that you want to separate by cation exchange chromatography. After binding to the column, you apply an increasing pH gradient to elute them (going from pH 0 to 14 for example). What is the order of elution of the amino acids (first, second, last)?
Gly, Glu, Lys
Gly, Lys, Glu
Lys, Gly, Glu
Glu, Gly, Lys