Would you expect the carotenoid pigment fraction to come, Chemistry

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Would you expect the carotenoid pigment fraction to come

In the chromatography of spinach extract, if you changed the eluent to 80:20 Hexane:acetone, what effect would this have on your separation? Would you expect the carotenoid pigment fraction to come off sooner or later than it did with 95:5 Hexane:Acetone? What effect would you expect if you used 100% hexane instead

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