Question- A cell extract containing all the enzymes of the Pentose Phosphate Pathway (and no other enzymes) was incubated with radioactive glucose in two experiments. Exp- 1) The glucose was labeled with carbon 14 in position 1. Radioactive carbon dioxide was produced. Exp- 2) The glucose was labeled with carbon 14 in position 2. No radioactive carbon dioxide was produced. a) Account for the different result in the two experiments. b) In experiment 2, which of the end products of the Pentose Phosphate Pathway, glyceraldehydes-3-phosphate and fructose-6-phosphate, would contain the radioactive carbon? Account for the molecules that would contain the radioactive carbon AND the specific location of the radioactive carbon in each labeled molecule.
Rework your solution from the beginning and check each step carefully.