Problem
I. When performing classic Sanger DNA sequencing, a reaction mixture must be prepared containing a specific combination of reagents and materials for each tube. List the 5 components of this mixture.
II. With classic Sanger DNA sequencing, a gel with four separate lanes for each nucleotide must be created. Why must each nucleotide have a separate lane?
III. Based on your answer in II, explain how Automatic Sequencing improved upon Sanger's method.