Discussion:
Microbiology Questions- Direct and Viable Counts
The depth of the hemocytometer sample box is 0.1mm for all questions.
Please show all calculations.
1. Why might the direct and variable counts of a suspension disagree?
2. You dilute an original sample 1:30. You then count the number of yeast in the 1:30 dilution. You count an average of 7 cells in the 1/25 mm^2 sized boxes. What is the density, in cells/ml, of the original sample?
3. You count the number of bacteria in 5 of the 1/400 mm^2 boxes of the central grid on the hemocytometer. Your results are: 12 cells in box #1, 17 cells in box #2, 17 cells in box #3, 14 cells in box #4 and 16 cells in box #5. You are counting a 1:10 dilution of the original sample. What is the density of the original culture (in cells/ml)?
Write your answer/response in detail with examples and facts and figures using APA style of formatting, size 12, font Times new roman and answer must be single spaced