Problem:
Question 1: Why is NAD+ free to diffuse within the mitochondrion whereas FAD+ is not ? What biochemical properties cause this difference ? Explain thoroughly.
Question 2: You need a solution containing 1000 buccal cells/mL. You count that you have 125 buccal cells in 50 uL from a total solution of 8 mL. How much of your stock solution should you take to make the 1000 cells/mL mixture? How much solvent if you would like a total volume of 5 mL? Show your calculations, but you do not need to write out any explanation.