Assignment Task: Watch the following video regarding gram staining protocol and answer the following questions:
YouTube Video: Gram Stain HD Animation
Q1. Why is a gram-stain called differential?
Q2. What is the purpose of passing the slide over the fire?
Q3. What is the first stain, what color does it stain?
Q4. For how long should the gram's iodine be left on the slide?
Q5. How are cells decolorized? Why is this step important?
Q6. If you were to see the cells under the microscope right after decolorization step, what color would you expect to see gram+ and gram- bacteria to be?
Q7. What is the name of the counterstain? What is the purpose of this stain?
Q8. Bacteria can be either gram + or gram -, what is the difference?
Q9. If a bacteria is gram +, what color would you expect to see under the microscope? (insert image)
Q9. If a bacteria is gram -, what color would you expect to see under the microscope? (insert image)
Q10. List five human diseases that are caused by bacteria. Include the name of the disease and the name of the bacteria that cause the disease.