Problem:
Answer the questions:
Question 1: You would like to identify an enzyme that could potentially be used in a biosensor to detect explosives. Describe a strategy by which you would find a new microbial enzyme that is active towards the explosive 2,4,6-trinitrotoluene (TNT).
Question 2: If we want to study a particular enzyme, why is it necessary to purify it ?
Question 3: Why do enzymes often require cofactors to facilitate activity ?