1. Which specific Mycobacterium organism was Ehrlich trying to stain when he developed the acid-fast stain?
2. Why is it best to use an older culture when performing the acid-fast stain?
3. What acid accumulates in the cells?
4. Who was Carl Weigert? What influence did Weigert have on Ehrlich?
5. Chemicals used in many staining procedures have characteristics that make them potentially harmful. What do the letters MSDS stand for? Follow the link provided to read about the acid-fast decolorizer. What kind of information does this document provide?
6. Other than the acid-fast stain, what other technique might be used to diagnose tuberculosis? What scientist developed this test?