Problem:
Question 1: Which DNA sequences must a synthetic gene contain in order for the gene to be inserted into the expression plasmid?
a) Origin of replication.
b) Restriction enzyme sites.
c) DNA ligase sites.
d) Ribosome binding site.
e) Lac operator.
Question 2: Which of the RNA molecules brings the new amino acids to the growing protein chain?
a) mRNA
b) tRNA
c) rRNA
d) all of the above
Question 3: Which of the following DNA sequences is a recognition site for restriction endonucleases:
a) AATT
b) ATTA
c) TTTT
d) AAAA
e) TAAT
Question 4: Is the following statement true of prokaryotic cells, eukaryotic cells or both? One gene can encode multiple different proteins (different in amino acid sequence).
a) Prokaryotic
b) Eukaryotic
c) Both prokaryotic and eukaryotic
Question 5: Assuming that the genes required for Isoleucine (an amino acid) biosynthesis are contained within an operon. If the cell is grown in the presence of high amounts of isoleucine you would expect to find:
a) The repressor protein to bind to the operator, preventing transcription.
b) The repressor protein to bind to the operator, allowing transcription.
c) The repressor protein released from the operator, allowing transcription.
d) The repressor protein released from the operator, preventing transcription.
Give reasoning with your answer.