Which restriction enzyme you use to clone dreadlocks gene


Problem

You are interested in working with a gene in fruit flies called dreadlocks. You design PCR primers to amplify a region that contains the dreadlocks gene, and you obtain the DNA shown below. The blue-colored region is the dreadlocks gene. Restriction enzyme sites are labeled, along with their base-pair positions. dreadlockimage.png Which restriction enzyme(s) should you use to clone the dreadlocks gene into the plasmid so you can use bacteria to replicate the plasmid + insert?

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