Problem
What would the effect of the following lab "errors" be on the outcome of each lab exercise specified below?
1) Genomic Lab - restriction analysis of purified genomic DNA: Intact bands were seen in lanes 5 (DNA + NspI) and 6 DNA + SacII) and fully digested bands were seen in Lanes 2 (DNA + H20), 3 (DNA + Buffer) and 4 (DNA + HaeIII). The photo of the gel from Microbacterium phage Hiddenleaf in Question 8 may be used for comparison. Offer an explanation for what might have occured?
2) Purification and Identification of Plasmid DNA: Plasmid miniprep DNA is left in isopropanol for 10 minutes before centrifugation step.
3) Restriction analysis of purified Genomic DNA: Comb with 8 teeth was used to make gel that only holds 15 uL per well.