Problem:
Imagine you have identified a putative transcription repressor protein. You hypothesise that the protein might bind to a specific region of DNA.You have performed an atomic force microscopy (AFM) experiment using purified protein and a 300 bp DNA fragment containing the putative binding region.
Required:
Question 1: What type of information can the AFM method provide?
Question 2: How would you know if the protein binds to more than one non-adjacent site on the DNA fragment?
Provide a brief explanation for your answer.