Problem:
I have a validated set of primers for rtPCR from Biorad that contains SYBR green.
Question: If I do rtPCR, can I use the rtPCR product after purifying it with a Qiagen PCR purification kit? Please justify your answer.
Also, I'm under the impression that the sequencing company needs primers to do sequencing,
Question: Do I just use the PCR primer or one of the "universal" primers the company has?