An enzyme catalyzed reaction is carried out in a 50 mLsolution containing 0.1 M TRIS buffer. The pH of the reactionmixture at the start was 8.0. As a result of the reaction, 00002mole of H+ were produced. What is the ratio of TRIS base to TRISacid at the start of the experiment?What is the ratio at the end of the experiment?