Problem:
Answer the questions:
Question 1: What specific genetic manipulation does Synechocystis readily allow that E. coli does not?
Question 2: For transformation of Synechocystis, why do you first plate your transformant mixture on a filter on a BG - 11 plate with glucose, and the next day the TA transfers the filter with cells to a plate that contains kanamycin (rather than plating directly on kanamycin)?
Question 3: What is the product of the psbC gene? What is its function?
Question 4: In the PCR reaction, what is the role of each of the PCR reagents: primers, dNTPs, Mg2+, and Taq polymerase?
Question 5: What are the considerations in determining the number of cycles needed for the PCR reaction?
Please explain all the answers in detail.