What is the most likely consequence of performing a scratch assay with cells overexpressing GFP, wild-type Cdc42 (WT-Cdc42), or a constitutively-active Cdc42 mutant that is energy independent (CA-Cdc42) when the Cdc42 GEF is rate limiting?
a. WT-Cdc42 and CA-Cdc42 both migrate slower than GFP
b.CA-Cdc42 migrates faster than GFP, whereas WT-Cdc42 migrates at the same speed as GFP
c. WT-Cdc42 and CA-Cdc42 both migrate faster than GFP
d. CA-Cdc42 migrates slower than GFP, whereas WT-Cdc42 migrates at the same speed as GFP
e. WT-Cdc42 and CA-Cdc42 both migrate at the same speed as GFP