Q1. If you mixed 8 mL of a 250,000 cells per mL culture with 56 mL of fresh broth, what is the final concentration?
Q2. You have a culture with an unknown cell density. You perform a dilution series of 10-2, 10-4 and 10-6 and plate 0.2 mL of each on nutrient agar plates. Only the 10-6 plate produces countable CFUs. There are a total of 495 CFUs on the plate. What was the original concentration of the unknown culture?
Q3. You have a culture with a cell density of 150,100 cell per mL. You take 3 mL of this culture and mix it with 22 mL of fresh medium. If you loaded this diluted culture onto a hemocytometer, how many cells should you see in one major square?