What is the difference between direct and indirect staining

Question

Question 1: List three reasons why you might choose to stain a slide rather than view it as unstained.

Question 2: Describe the following terms in full detail in your own words:

• Acidic Dye:
• Basic Dye:
• Direct Stain:
• Indirect Stain:

Question 3: What is the difference between direct and indirect staining?

Question 4: What is heat fixing?

Question 5: Why is it necessary to ensure that your specimens are completely air dried prior to heat fixing?

Question 6: How do direct stains differ from indirect stains in terms of ionic composition and their interactions with cell surfaces, i.e., how they work?

Question 7: Why is it important to form bacterial smears by mixing cells with a small amount of liquid and spreading them over a slide surface?

Question 8: What are two functions of heat-fixing bacterial smears prior to staining?

Question 9: When is negative staining used?

Question 10: List the three basic shapes of bacteria.

Question 11: List and describe 5 different arrangements of cocci.