what is the determination of vitamin b1vitamin b1, Biology

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what is the determination of vitamin b1vitamin b1

What is the Determination of vitamin B₁

Vitamin B₁ is determined by chemical method and microbiological assay. In the chemical method, vitamin B₁ is oxidized in alkaline solution by potassium ferricyanide solution to form thiochrome. The latter may be extracted with isobutanol and the resulting vivid blue fluorescence measured

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