The phosphofructokinase reaction in GLYCOLYSIS is: Fructose-6-P + ATP ? Fructose-1,6-bisphosphate + ADP + H+ The standard state free energy change for this reaction is -14,200 J/mol. a) Calculate the standard state free energy of HYDROLYSIS of fructose-1,6-bisphosphate to fructose-6-P.. (I think here ATP hydrolysis is -30.5kj/mole). I'm so confused between glycolysis and hydrolysis. b) The human red blood cell concentrations of various metabolites are: [ATP] = 1.85 mM, [ADP] = 0.14 mM, [fructose-6-P] = 0.014 mM, [fructose-1,6-bisphosphate] = 0.031 mM What is the cellular free energy change for the phosphofructokinase reaction at 37°C? c) What is the equilibrium constant for the phosphofructokinase reaction?