Discussion:
KM of a Michaelis-Menten enzyme
1. The KM of a Michaelis-Menten enzyme for a substrate is 1.0 x 10-4 M. At a substrate concentration of 0.2 M, Vo=43x10^-6 M for a certain enzyme concentration. However, with a substrate concentration of 0.02 M, Vo has the same value.
(A) Using numerical calculations show that this observation is accurate.
(B) What is the best range of [S] for measuring KM?
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