Problem:
In the nematode C. elegans, sup-5 is a Trp tRNA gene. Mutant alleles of sup-5 function as an amber (tRNA) suppressor.
Required:
Question 1: What is the anticipated sequence change to the DNA sequence of the sup-5 gene that would be associated with the suppressor (mutant) allele? Indicate the sequence of the double stranded DNA, as well as which strand is the template strand for transcription (or, the direction of transcription of the sup-5 gene).
Question 2: Animals homozygous for the suppressor allele of sup-5 are viable. What does this suggest about the genome of C. elegans?
so any suggestions should ideally aid in this goal.