Problem:
I am conducting a biochemistry-related experiment and I have been unable to understand a step which is commonly performed. My aim in this step is to apply a PEG (Polyethylene glycol) silane layer.
After immersing indium tin oxide (ITO) slides in a PEG-concentration, the slides are incubated for 18 hours at a temperature of 60 degrees Celsius.
Question: Can you tell me why the incubation is performed? And why for 18 hours and at 60 degrees Celsius? Please provide your answers.