What is nicked circular dna and why is it termed relaxed
Problem:
Question 1: Alcohol is considered to be a significant reagent for the isolation for nucleic acid (RNA and DNA). Why is this so?
Question 2: What is nicked circular DNA and why is it termed "relaxed"?
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What is nicked circular DNA and why is it termed "relaxed"?
why was DNA digestions carried out in 370c?
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what could account for low end nuclease activity?
Why are the restriction enzymes added last to the digestion mixtures?
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