Problem:
The bacteriophage lambda genome can exist in either a linear form or a circular form. The circular form occurs when the 20-bp cos sites (cohesive ends) anneal at their complementary base pairs and are ligated.
Required:
Question 1: How many fragments will be formed by restriction enzyme digestion with XhoI in the linear form of the ? genome? - 1,2,3, or 4
Question 2: How many fragments will be formed by restriction enzyme digestion with XbaI in the circular form of the ? genome? 1,2,3, or 4
Question 3: How many fragments will be formed by restriction enzyme digestion with both XhoI and XbaIin the circular form of the ? genome? 1,2,3, or 4
Please describe all the answers.