Q1. The sample(s) that you added to the microplate strip contain many proteins and may or may not contain the disease antigen. What happened to the proteins in the plastic well if the sample contained the antigen? What if it did not contain the antigen?
Q2. Why did you need to wash the wells after each step? What would happen if you did not properly wash?
Q3. ???If the sample gave a negative result for the disease-causing agent, does this mean that you do not I the disease? What reasons) could there be for a negative result when you actually do have the disease?
Q5. Why did you assay your samples in triplicate?
Q6. When you added the secondary antibody, what happened if your serum sample was positive? What if it was negative?
Q7. Why is it important to be able to detect antibodies in people, especially in people who don't appear sick?
Q8. Why or how are enzymes used in this immunoassay?
Q9. The controls of an ELISA test react correctly (both positive and negative controls). The patient sample only reacts in the undiluted (1:1) sample well (none of the dilutions are positive - 1:2, 1:4, 1:8). What is the titer of the patient sample?
Q10. If the positive control does not show a positive reaction, how do you interpret the patient samples?
Q11. If the negative control shows a weak positive reaction while the positive control shows a strong positive reaction, what should you do next?
Q12. Outline the steps you would take to test for Direct ELISA for COVID-19 in a patient sample. What would you place in the wells First, Second, Third, Fourth? Be specific-(ie., if using a secondary antibody, what animal is it from?)