Problem:
The genome of virus SV40 is a circular double? Stranded DNA molecule 5 kb long (i.e., it contains 10,000 bases).
a. A 1 kb region is amplified by PCR for four cycles. (Assume that specific amplification of the target region occurs.) What fraction of the total DNA does the target sequence constitute? Show your work.
b. After many generations of growth in medium containing 14N, the medium was replaced with new medium containing 15N. After five generations, what is the ratio of light: heavy DNA strands? Show your work.
Please describe the work done for the mentioned problem.