Problem:
Question: What enzyme or function is being affected by each mutation of the mutations listed at the nonpermissive temperature.
1) no peptide bond is formed between the amino acid bound to the tRNA at the A site and the nascent polypeptide attached to the tRNA at the P site.
2) completed polypeptide is not released from the ribosome
3) fmet.tRNA-fMet is not binding to the Psite
4) translocation does not occur
5) aminoacylated tRNAs are not binding at the A site
Please select correct answer and also provide explaination.