Problem
1. What enzyme did you choose to make your recombinant plasmid & why?
2. Would it be possible to select a different enzyme (based on this plasmid map), why or why not?
3. Which colonies (by color) would you choose to purify the protein that was expressed from your Alzheimer gene? Why did you choose these colonies?
4. IF you knew that a desired protein made by the transformants was negative what specific chromatography (exchanger) would you use to obtain your negatively charged molecule first off the column.