What does is meant by the term sticky ends


Question 1: Why are both the DNA constructs and plasmid vectors digested by the same restriction enzymes in this experiment?

Question 2: What does is meant by the term "sticky ends"

Question 3: You have a linear piece of DNA shown below and treat it with the restriction enzyme EcoRI (*see lab assignment for EcoRI recognition site).

GCGGTTACAAGAATTCCGCGCCCTATATTTGAATTCACCTGAC CGCCAATGTTCTTAAGGCGCGGGATATAAACTTAAGTGGACTG

a. How many DNA fragments will result after EcoRI cuts this piece of DNA?

b. Draw the DNA fragments and label the sticky ends.

Question 4: How do we make cells "competent" for transformation?

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Biology: What does is meant by the term sticky ends
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