Q1. if a mixture of compounds of widely different volatilities is to be analysed by gas chromatography ,what sort of temperture regime can be employed to optimise separation and resolution ?
Q2. Interferences are often a problem in atomic spectoscopy .Which type of interference sometimes arises in the atomic spectrometric determination of sodium and which type sometimes arises in the atomic spectometric determination of calcium?
Q3. why do UV visible spectrophotometers have two light sources?
Q4. why would be difficult to analyse glucose directly using HPLC with UV detection?
Q5. Name a technique that is based upon electrophoresis, which can be used to determine the RMM of a protein?
Q6. what colour is the light absorbed by Biuret reaction prodct?
Q7. Give the structure of the part of protein molecule that reacts with the Biuret reagent in the Biuret reaction?