1. Urine was collected from a person known to have ingested "a lot of aspirin". Aspirin (acetylsalicylic acid) is known to be rapidly hydrolyzed in the body to salicylic acid that is then further metabolized to salicyluric acid (50-80%), salicyl 0-glucuronide (10-30%), and free salicylic acid (5-10%). In general terms, what would you have to do in order to extract the urine for quantitation of ingested aspirin?
2. If extraction of cocaine is 80% efficient and GCMS quantitation shows that 150 mg of cocaine is present in the extracted sample, how much cocaine was present in the original sample?
3. What are the pros and cons for using liquid-liquid extraction?
4. What are the pros and cons for using SPE?