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Two isozymes mediate the conversion of glucose glucose

Two isozymes mediate the conversion of Glucose Glucose 6-PO4. Hexokinase has a Km of 23nM for Glucose, while Glucokinase has a Km of 97nM for Glucose.

If I had a mixture of the two enzymes, could I use an affinity column with Glucose as the ligand to separate the two proteins? If so, how? If not, why not?

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